RNA endotoxin hydrophobic interaction ionic recognition multimodal chromatography plasmid DNA. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. Capto adhere is a multimodal strong anion exchanger for BioProcess applications. In addition to the removal of typical product and process-related impurities, chromatography steps are also used to remove potential adventitious viral contaminants and non-infectious retrovirus-like particles expressed by rodent cell lines used for production. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Capto Adhere proved to offer a very strong binding of nucleic acids. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. directly through the column while the contaminants are adsorbed. L (600/200) ReadyToProcess Capto adhere columns in operation with water at room temperature (20C). The most pronounced are ionic interaction, hydrogen bonding, and hydrophobic interaction. Chromatography with Capto Adhere in bindingmode with elution by linear salt gradient at pH 7.5 resulted in optimal yield, purity and HCP reduction factor. Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The Capto adhere ligand, N-benzyl-N-methyl ethanolamine, exhibits many functionalities for interaction.
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